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rabbit phospho ser cdks substrate p s2 100 cell signaling technology 9477s  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit phospho ser cdks substrate p s2 100 cell signaling technology 9477s
    Rabbit Phospho Ser Cdks Substrate P S2 100 Cell Signaling Technology 9477s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+ser+cdk+substrate/pm39232161-1037-349-354?v=Cell+Signaling+Technology+Inc
    Average 94 stars, based on 85 article reviews
    rabbit phospho ser cdks substrate p s2 100 cell signaling technology 9477s - by Bioz Stars, 2026-08
    94/100 stars

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    Figure 1. Determining the Interphase and Mitotic F-Actin Interactomes (A) Schematic representation of the synchronization procedure and the experimental pipeline. Cells were first arrested in S phase by thymidine treatment. For synchronization in interphase, thymidine-pre-treated cells were then released and treated with the <t>CDK1</t> inhibitor RO3306, yielding a population of cells in G2 phase. For synchronization in metaphase, thymidine-pre-treated cells were submitted to a short treatment with nocodazole, followed by a mitotic shake off and metaphase arrest with the proteasome inhibitor MG132. The drugs used for synchronization were washed out prior to extract preparation. (B) Representative images of HeLa cells after synchronization following the procedure described in (A). Cells were stained with DAPI (red) to visualize DNA and phalloidin (cyan) to visualize F-actin. Scale bars, 10 mm. (C) Immunoblot of actin, cyclin B, and phospho-Histone H3 (P-HH3) levels in the different synchronized cell extracts. S, supernatant; P, Pellet.
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    Figure 1. Determining the Interphase and Mitotic F-Actin Interactomes (A) Schematic representation of the synchronization procedure and the experimental pipeline. Cells were first arrested in S phase by thymidine treatment. For synchronization in interphase, thymidine-pre-treated cells were then released and treated with the <t>CDK1</t> inhibitor RO3306, yielding a population of cells in G2 phase. For synchronization in metaphase, thymidine-pre-treated cells were submitted to a short treatment with nocodazole, followed by a mitotic shake off and metaphase arrest with the proteasome inhibitor MG132. The drugs used for synchronization were washed out prior to extract preparation. (B) Representative images of HeLa cells after synchronization following the procedure described in (A). Cells were stained with DAPI (red) to visualize DNA and phalloidin (cyan) to visualize F-actin. Scale bars, 10 mm. (C) Immunoblot of actin, cyclin B, and phospho-Histone H3 (P-HH3) levels in the different synchronized cell extracts. S, supernatant; P, Pellet.
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    Figure 1. Determining the Interphase and Mitotic F-Actin Interactomes (A) Schematic representation of the synchronization procedure and the experimental pipeline. Cells were first arrested in S phase by thymidine treatment. For synchronization in interphase, thymidine-pre-treated cells were then released and treated with the CDK1 inhibitor RO3306, yielding a population of cells in G2 phase. For synchronization in metaphase, thymidine-pre-treated cells were submitted to a short treatment with nocodazole, followed by a mitotic shake off and metaphase arrest with the proteasome inhibitor MG132. The drugs used for synchronization were washed out prior to extract preparation. (B) Representative images of HeLa cells after synchronization following the procedure described in (A). Cells were stained with DAPI (red) to visualize DNA and phalloidin (cyan) to visualize F-actin. Scale bars, 10 mm. (C) Immunoblot of actin, cyclin B, and phospho-Histone H3 (P-HH3) levels in the different synchronized cell extracts. S, supernatant; P, Pellet.

    Journal: Developmental cell

    Article Title: F-Actin Interactome Reveals Vimentin as a Key Regulator of Actin Organization and Cell Mechanics in Mitosis.

    doi: 10.1016/j.devcel.2019.12.011

    Figure Lengend Snippet: Figure 1. Determining the Interphase and Mitotic F-Actin Interactomes (A) Schematic representation of the synchronization procedure and the experimental pipeline. Cells were first arrested in S phase by thymidine treatment. For synchronization in interphase, thymidine-pre-treated cells were then released and treated with the CDK1 inhibitor RO3306, yielding a population of cells in G2 phase. For synchronization in metaphase, thymidine-pre-treated cells were submitted to a short treatment with nocodazole, followed by a mitotic shake off and metaphase arrest with the proteasome inhibitor MG132. The drugs used for synchronization were washed out prior to extract preparation. (B) Representative images of HeLa cells after synchronization following the procedure described in (A). Cells were stained with DAPI (red) to visualize DNA and phalloidin (cyan) to visualize F-actin. Scale bars, 10 mm. (C) Immunoblot of actin, cyclin B, and phospho-Histone H3 (P-HH3) levels in the different synchronized cell extracts. S, supernatant; P, Pellet.

    Article Snippet: The following primary antibodies were used:mouse anti-cyclin B (Santa Cruz Biotechnology sc-245; 1/1000); mouse anti-b-actin (Santa Cruz Biotechnology sc-47778; 1/1000); mouse anti-GAPDH (Abcam ab8245; 1/4000); phosphoHistone H3 S10 (Cell Signaling Tech 9701; 1/1000); mouse anti-MRLC pS19 (Cell Signaling Tech 3675; 1/1000); rabbit anti-MRLC (Cell Signaling Tech 3672; 1/1000); rabbit anti-vimentin (Cell Signaling Tech 3932; 1/1000); mouse anti-plectin (Thermo Fisher Scientific PA556292 1/1000); mouse anti-a-tubulin (ThermoFisher Scientific DM1A; 1/1000); rabbit-anti phospho (Ser) CDK1 substrate (Cell Signaling Tech 9477; 1/1000); rat anti-GFP (Chromotek; 1/1000); rabbit anti-VASP (Cell Signaling Tech 3132S; 1/1000); rabbit anti-MENA (Santa Cruz Biotechnology sc-135988; 1/1000); mouse anti-MYH9 (Abnova; 1/1000) ; mouse anti-caldesmon (Abcam ab183146; 1/1000); mouse anti-fascin (Santa Cruz Biotechnology sc-21743; 1/1000), rabbit anti-filamin (Cell Signaling Tech 4762S; 1/1000), rabbit anti-cofilin (Cell Signaling Tech 3318S; 1/1000) and WDR1 (Santa Cruz Biotechnology sc-135988; 1/500).

    Techniques: Staining, Western Blot